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Elisabeth Holen

elisabeth
Forsker
Telefon: 95241633
Avdeling: Fôr og ernæring

Publikasjoner

Vitenskapelige artikler (NVI)

2023

Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid

Pedro Araujo, Sarah Iqbal, Aleksander Arnø, Marit Espe, Elisabeth Holen
Molecules 28
2022

Optimization of the Balance between Protein, Lipid and Carbohydrate in Diets for Lumpfish (Cyclopterus lumpus)

Kristin Hamre, Gerd Marit Berge, Øystein Sæle, Elisabeth Holen, Katerina Kousoulaki, Sofie C. Remø, Åshild Krogdahl, Ingrid Lein
Aquaculture Nutrition p. 1-15
2022

Dietary electrolyte balance of Atlantic salmon (Salmo salar) freshwater feeds: Impact on osmoregulation, mineral metabolism and performance in seawater

P. Antony Jesu Prabhu, Per Gunnar Fjelldal, Sofie C. Remø, Chandrasekar Selvam, Kristin Hamre, Marit Espe, Elisabeth Holen, Kaja Helvik Skjærven, Vibeke Vikså, Saravanan Subramanian, Johan W. Schrama, Nini Sissener
Aquaculture 546
2022

Tailoring freshwater diets towards boosted immunity and pancreas disease infection robustness in Atlantic salmon post smolts

Elisabeth Holen, M Chen, Per Gunnar Fjelldal, Kaja Helvik Skjærven, Nini Sissener, Sofie C. Remø, P. Antony Jesu Prabhu, Kristin Hamre, Vibeke Vikeså, S. Subramanian, Marit Espe
Fish and Shellfish Immunology 120 p. 377-391
2022

Dietary plant oil supplemented with arachidonic acid and eicosapentaenoic acid affects the fatty acid composition and eicosanoid metabolism of Atlantic salmon (Salmo salar L.) during smoltification

L. Miao, Sofie C. Remø, Marit Espe, P. Antony Jesu Prabhu, Kristin Hamre, Per Gunnar Fjelldal, Kaja Helvik Skjærven, Elisabeth Holen, Vibeke Vikeså, Nini Sissener
Fish and Shellfish Immunology 123 p. 194-206
2022

Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua)

Elisabeth Holen, Marit Espe, Anett Kristin Larsen, Pål Asgeir Olsvik
Fish and Shellfish Immunology 127 p. 549-560
2021

RNA form baker's yeast cultured with and without lipopolysaccharide (LPS) modulates gene transcription in an intestinal epithelial cell model, RTgutGC from rainbow trout (Oncorhynchus mykiss)

Elisabeth Holen, Maren Hoff Austgulen, Marit Espe
Fish and Shellfish Immunology 119 p. 397-408
2021

Differential production of prostaglandins and prostacyclins by liver and head kidney cells from Atlantic salmon challenged with arachidonic and eicosapentaenoic acids

Hernandez Pedro W Araujo, Marit Espe, Eva Lucena, Yang Yang, Elisabeth Holen
2021

Leucine did not stimulate growth and accretion in either stressed or unstressed Atlantic salmon

Marit Espe, Floriana Lai, Ana S. Gomes, Christian De Santis, Mali B. Hartviksen, Elisabeth Holen, Ivar Rønnestad
Aquaculture Nutrition 27 p. 2459-2467
2021

Can improved nutrition for Atlantic salmon in freshwater increase fish robustness, survival and growth after seawater transfer?

Nini Sissener, Kristin Hamre, Per Gunnar Fjelldal, P. Antony Jesu Philip, Marit Espe, L. Miao, Erik Höglund, Christina Sørensen, Kaja Skjærven, Elisabeth Holen, S. Subramanian, V. Vikeså, Birgitta Norberg, Sofie Remø
Aquaculture 542
2020

Dietary selenium required to achieve body homeostasis and attenuate pro-inflammatory responses in Atlantic salmon post-smolt exceeds the present EU legal limit

P. Antony Jesu Prabhu, Elisabeth Holen, Marit Espe, Marta Sofia Silva, May-Helen Holme, Kristin Hamre, Erik-Jan Lock, Rune Waagbø
Aquaculture 526:735413 p. 1-14
2020

The level of 1C diets fed prior to cell isolation affects lipid metabolism in primary liver cells isolated from Atlantic salmon (Salmo salar)

Marit Espe, Kaja Helvik Skjærven, Ming Chen, Vibeke Vikeså, Anne-Catrin Adam, Takaya Saito, Elisabeth Holen
Aquaculture Nutrition 26 p. 1019-1025
2020

Surplus spermine does not protect salmon cells against lipopolysaccharide (LPS)-induced stress, but excess spermine is eliminated through spermidine/spermine N1-acetyltransferase (SSAT)

Marit Espe, Shiwei Xie, Shijun Chen, Anders Aksnes, Elisabeth Holen
Aquaculture Nutrition 26 p. 946-953
2019

Resveratrol inhibited LPS induced transcription of immune genes and secretion of eicosanoids in Atlantic salmon (Salmo salar), comparing mono-, co- and a novel triple cell culture model of head kidney leukocytes, liver cells and visceral adipocyte tissue

Elisabeth Holen, Pedro Araujo, Shiwei Xie, Liv Ingeborg Rosvoll Søfteland, Marit Espe
224
2019

Effect of dietary replacement of fish meal with insect meal on in vitro bacterial and viral induced gene response in Atlantic salmon (Salmo salar) head kidney leukocytes

Oda Kvalsvik Stenberg, Elisabeth Holen, Luisa Piemontese, Nina Sylvia Liland, Erik-Jan Lock, Marit Espe, Ikram Belghit
Fish and Shellfish Immunology 91 p. 223-232
2019

The effect of omega-3 and omega-6 polyunsaturated fatty acids on the production of cyclooxygenase and lipoxygenase metabolites by human umbilical vein endothelial cells

Pedro Araujo, Ikram Belghit, Niels Aarsaether, Marit Espe, Eva Lucena, Elisabeth Holen
Nutrients 11:966 p. 1-18
2019

Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin

Marit Espe, Shiwei Xie, Shijun Chen, Pedro Araujo, Elisabeth Holen
Aquaculture Nutrition 25 p. 737-746
2019

Surplus arginine reduced lipopolysaccharide induced transcription of proinflammatory genes in Atlantic salmon head kidney cells

Graciela P Martins, Marit Espe, Zhihao Zhang, Igo Gomes Guimarães, Elisabeth Holen
Fish and Shellfish Immunology 86 p. 1130-1138
2018

Extraction of Pro- and Anti-Inflammatory Biomarkers from fish Cells Exposed to Polyunsaturated Fatty Acids and Quantification by Liquid Chromatography Tandem Mass Spectrometry

Eva Lucena, Yang Yang, Cioly Mendez, Elisabeth Holen, Hernandez Pedro W Araujo
Current Analysis on Chemistry
2018

A comparative study: Difference in omega-6/omega-3 balance and saturated fat in diets for Atlantic salmon (Salmo salar) affect immune-, fat metabolism-, oxidative and apoptotic-gene expression, and eicosanoid secretion in head kidney leukocytes

Elisabeth Holen, Pedro Araujo, Nini Sissener, Grethe Rosenlund, Rune Waagbø
Fish and Shellfish Immunology 72 p. 57-68
2017

Choline supplementation increased total body lipid gain, while surplus methionine improved growth and amino acid retention in adult Atlantic salmon (Salmo salar)

Marit Espe, Synne Marte Andersen, Eva Veiseth-Kent, Ivar Rønnestad, Elisabeth Holen, Jens-Erik Zerrahn, Anders Aksnes
Aquaculture Nutrition 23 p. 1086-1094
2017

Phytosterols are not involved in the development of fatty liver in plant oil fed Atlantic salmon (Salmo salar) at high or low water temperature

Nini Sissener, Nina Sylvia Liland, Elisabeth Holen, Ingunn Stubhaug, Bente Elisabeth Torstensen, Grethe Rosenlund
Aquaculture 480 p. 123-134
2016

β-naphthoflavone interferes with cyp1c1, cox2 and IL-8 gene transcription and leukotriene B<sub>4</sub> secretion in Atlantic cod (Gadus morhua) head kidney cells during inflammation

Elisabeth Holen, Pål Asgeir Olsvik
Fish and Shellfish Immunology 54 p. 128-134
2016

Choline supplementation to low methionine diets increase phospholipids in Atlantic salmon, while taurine supplementation had no effects on phospholipid status, but improved taurine status

Marit Espe, Jens-Erik Zerrahn, Elisabeth Holen, Ivar Rønnestad, Eva Veiseth-Kent, Anders Aksnes
Aquaculture Nutrition 22 p. 776-785
2016

β-naphthoflavone interferes with cyp1c1, cox2 and IL-8 gene transcription and leukotriene B4 secretion in Atlantic cod (Gadus morhua) head kidney cells during inflammation

Elisabeth Holen, Pål Asgeir Olsvik
Fish and Shellfish Immunology 54 p. 128-134
2016

Hydrolyzed fish proteins modulates both inflammatory and antioxidant gene expression as well as protein expression in a co culture model of liver and head kidney cells isolated from Atlantic salmon (Salmo salar)

Elisabeth Holen, Juyun He, Pedro Araujo, Jørgen Seliussen, Marit Espe
Fish and Shellfish Immunology 54 p. 22-29
2015

Adult Atlantic salmon (Salmo salar L.) adapts to long-term surplus dietary arginine supplementation

Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe
Aquaculture Nutrition 21 p. 355-363
2015

Combining eicosapentaenoic acid, decosahexaenoic acid and arachidonic acid, using a fully crossed design, affect gene expression and eicosanoid secretion in salmon head kidney cells invitro

Elisabeth Holen, Juyun He, Marit Espe, Liqiou Chen, Pedro Araujo
Fish and Shellfish Immunology 45 p. 695-703
2015

Hydrolyzed fish proteins reduced activation of caspase-3 in H 2 O 2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar)

Marit Espe, Elisabeth Holen, Juyun He, Fiona Provan, Liqiao Chen, Kjell Birger Øysæd, Jørgen Seliussen
SpringerPlus 4
2014

The impact of exogenous ω-6 and ω-3 polyunsaturated fatty acids on the induced production of pro- and anti-inflammatory prostaglandins and leukotrienes in Atlantic salmon head kidney cells using a full factorial design and LC-MS/MS

Pedro Araujo, Eva Lucena, Yang Yang, Bupesh Ceemala, Zebasil Tassew Mengesha, Elisabeth Holen
Journal of chromatography. B 964 p. 164-171
2014

A co culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling

Elisabeth Holen, Marit Espe, Synne Marte Andersen, Richard Taylor, Anders Aksnes, Zebasil Tassew Mengesha, Pedro Araujo
Fish and Shellfish Immunology 37 p. 286-298
2014

Arginine supplementation and exposure time affects polyamine and glucose metabolism in primary liver cells isolated from Atlantic salmon

Synne Marte Andersen, Richard Taylor, Elisabeth Holen, Anders Aksnes, Marit Espe
Amino Acids 46 p. 1225-1233
2014

Aryl hydrocarbon receptor protein and Cyp1A1 gene induction by LPS and phenanthrene in Atlantic cod (Gadus morhua) head kidney cells

Elisabeth Holen, Pål Asgeir Olsvik
Fish and Shellfish Immunology 40 p. 384-391
2014

Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon

Marit Espe, Synne Marte Andersen, Elisabeth Holen, Ivar Rønnestad, Eva Veiseth-Kent, Jens-Erik Zerrahn, Anders Aksnes
British Journal of Nutrition 112 p. 1274-1285
2013

Cytokine gene expression and prostaglandin production in head kidney leukocytes isolated from Atlantic cod (Gadus morhua) added different levels of arachidonic acid and eicosapentaenoic acid

Miriam Furné Castillo, Elisabeth Holen, Pedro Araujo, Kai Kristoffer Lie, Mari Moren
Fish and Shellfish Immunology 34 p. 770-777
2013

Taurine attenuates apoptosis in primary liver cells isolated from Atlantic salmon (Salmo salar)

Marit Espe, Elisabeth Holen
British Journal of Nutrition 110 p. 20-28
2013

Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar)

Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe
British Journal of Nutrition 110 p. 1968-1977
2012

Low impact of exposure to environmentally relevant doses of Ra-226 in Atlantic cod (Gadus morhua) embryonic cells

Pål Asgeir Olsvik, Marc Berntssen, Ketil Hylland, Dag Ø. Eriksen, Elisabeth Holen
Journal of Environmental Radioactivity 109 p. 84-93
2012

Pathogen recognition and mechanisms in Atlantic cod (Gadus morhua) head kidney cells Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes

Elisabeth Holen, Kai Kristoffer Lie, Hernandez Pedro W. Araujo, Pål Asgeir Olsvik
Fish and Shellfish Immunology 33 p. 267-276
2012

Application of Doehlert uniform shell designs for selecting optimal amounts of internal standards in the analysis of prostaglandins and leukotrienes by liquid chromatography-tandem mass spectrometry

Hernandez Pedro W. Araujo, Steve Janagap, Elisabeth Holen
Journal of Chromatography A 1260 p. 102-110
2011

Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon (Salmo salar) fed soy bean oil or fish oil based diets

Elisabeth Holen, Synnøve Winterthun, ZHEN-YU DU, Anne Vatland Krøvel
Fish and Shellfish Immunology 30 p. 397-405
2011

Ontogenetic expression of maternal and zygotic genes in Atlantic cod embryos under ambient and thermally stressed conditions

Kaja Helvik Skjærven, Pål Asgeir Olsvik, Roderick Nigel Finn, Elisabeth Holen, Kristin Hamre
Comparative Biochemistry and Physiology A 159 p. 196-205
2011

Development of a co-culture model for in vitro toxicological studies in Atlantic salmon

Anne Vatland Krøvel, Synnøve Winterthun, Elisabeth Holen, Pål Asgeir Olsvik
Toxicology in Vitro 25 p. 1143-1152
2011

Transcriptional evidence for low contribution of oil droplets to acute toxicity from dispersed oil in first feeding Atlantic cod (Gadus morhua) larvae

Pål Asgeir Olsvik, Bjørn Henrik Hansen, Trond Nordtug, Mari Moren, Elisabeth Holen, Kai Kristoffer Lie
154 p. 333-345
2010

Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2

Elisabeth Holen, Anette Kausland, Kaja Helvik Skjærven
36 p. 1029-1039
2010

Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua) - Effects of BNF, PCDD and Cd

Liv Ingeborg Rosvoll Søfteland, Elisabeth Holen, Pål Asgeir Olsvik
151 p. 401-411
2008

Direct injection of redissolved cell culture media into a single-column liquid chromatography coupled to mass spectrometry for the measurement of PGE2

Hernandez Pedro W Araujo, Zhen-yu Du, Thu Thao Nguyen, Elisabeth Holen
Open Analytical Chemistry Journal
2007

Pigmentation and eye-migration in Atlantic halibut (Hippoglossus hippoglossus L.) larvae: Relevance of knowledge on development of pigment cells and determination of body axes in vertebrates.

Kristin Hamre, Elisabeth Holen, Mari Moren
Aquaculture Nutrition 13 p. 65-80
2005

Dietary nucleotides and human immune cells. II. Modulation of PBMC growth and cytokine secretion

Elisabeth Holen, Oddvin Anton Bjørge, Roland Jonsson
Nutrition Research 21 p. 1003-1009
2004

Dietary nucleotides and intestinal cell lines: I. Modulation of growth

Elisabeth Holen, Roland Jonsson
Nutrition Research 24 p. 197-207
1999

Horfor øker hyppigheten av allergi i industrialiserte land mer enn i utviklingsland?

Elisabeth Holen, Said Elsayed
? 119 p. 3176-3177
1999

Evaluating B and T cell epitopes of ovomucoid: Intact modified molecules, CNBr fragments and synthetic peptides

Said Elsayed, Elisabeth Holen
Allergy. European Journal of Allergy and Clinical Immunology 54 p. 211
1999

Characterization of T cell response to a-lactalbumin in PMMC cultures from cow mild hypersensitivity adults

Said Elsayed, Elisabeth Holen
Allergy. European Journal of Allergy and Clinical Immunology 54 p. 422

Annet tidsskriftbidrag

2023

Editorial: Immunity and disease of aquatic organisms under the combined impact of anthropogenic stressors: mechanisms and disease outcomes

Anett Kristin Larsen, Elisabeth Holen, Helmut Segner
Frontiers in Marine Science 10
2018

Corrigendum to “A comparative study: Difference in omega-6/omega-3 balance and saturated fat in diets for Atlantic salmon (Salmo salar) affect immune-, fat metabolism-, oxidative and apoptotic-gene expression, and eicosanoid secretion in head kidney leukocytes” [Fish Shellfish Immunol. 72 (2018) 57–68](S1050464817306502)(10.1016/j.fsi.2017.10.040)

Elisabeth Holen, Pedro Araujo, Nini Sissener, Grethe Rosenlund, Rune Waagbø
Fish and Shellfish Immunology 83 p. 449
2013

Enhanced polyamine turnover after arginine supplementation in an Atlantic salmon model-implications on growth and animal welfare

Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe
Amino Acids 45 p. 575-576
2013

Methionine limitation-a threat to health? Using Atlantic salmon as the model

Marit Espe, Synne Marte Andersen, Elisabeth Holen, Jens-Erik Zerrahn, Ivar Rønnestad, E. Veiseth, Anders Aksnes
Amino Acids 45 p. 591-591

Faglige foredrag

2021

Balansert sammensetning av makronæringsstoffer i fôr til rognkjeks (Cyclopterus lumpus)

Kristin Hamre, Gerd Marit Berge, Elisabeth Holen, Øystein Sæle, Katerina Kousoulaki, Sofie C. Remø, Åshild Krogdahl, Ingrid Lein
2021

Surplus methionine, cobalamin, folate and pyridoxine improved growth and lipid metabolism in Atlantic salmon

Marit Espe, Elisabeth Holen, Anne-Catrin Adam, Takaya Saito, Kaja Helvik Skjærven
2019

Trenger laks fettsyren EPA for optimal immunrespons?

Nina Sylvia Liland, Bjørg-Kristine Hundal, Elisabeth Holen, Marit Espe, Sofie C. Remø, Ingunn Stubhaug, Grethe Rosenlund, Nini Sissener
2018

Effekter av resveratrol på lipo-polysakkarid indusert transkripsjon av immungener og eikosanoidsekresjon i atlantisk laks, ved bruk av forskjellige cellemodeller.

Elisabeth Holen, Pedro Araujo, S Xie, Liv Ingeborg Rosvoll Søfteland, Marit Espe
2017

Omega-6/omega-3 balanse og metta feitt i fôr til laks – konsekvensar for immunrespons og sjukdomsforløp av PD?

Sofie c Remø, Nini Hedberg Sissener, Elisabeth Holen, Sonal Jayesh Patel, Sven Martin Jørgensen, Hernandez Pedro W Araujo, Ingrid Uglenes Fiksdal, Charles McGurk, Grethe Rosenlund, Bente Elisabeth Torstensen, Rune Waagbø
2016

Omega-6/Omega-3 balance and saturated fat in diets for Atlantic salmon- effects on fish health and disease resistance

Sofie c Remø, Nini Hedberg Sissener, Elisabeth Holen, Sonal Jayesh Patel, Sven Martin Jørgensen, Hernandez Pedro W Araujo, Inger Uglenes, Charles McGurk, Grethe Rosenlund, Bente Elisabeth Torstensen, Rune Waagbø
2016

In Vitro Fisk: Nye 3D modeller til ernæring og toksikologisk forskning.

Liv Ingeborg Rosvoll Søfteland, Elisabeth Holen, Marit Espe, Pål Asgeir Olsvik
2014

Kan arginintilsetning påvirke forbrenning av visceralt fett gjennom økt polyaminomsetning i laks?

Synne Marte Andersen, Elisabeth Holen, Ivar Rønnestad, Jan Erik Zerrahn, Richard Taylor, Anders Aksnes, Marit Espe
2013

Do diets with 1-5% EPA and DHA induce inflammation in Atlantic salmon?

Nina Sylvia Liland, Elisabeth Holen, Bente Elisabeth Torstensen, Nini Sissener, Grethe Rosenlund
2012

Is PGE2 production dependent on the EPA:ARA ratio or only the concentration of ARA? In vitro studies with primary cell cultures from A. cod head kidney

Miriam Furné Castillo, Hernandez Pedro W Araujo, Elisabeth Holen, Mari Moren, Kai Kristoffer Lie
2012

Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect different genes in Atlantic cod head kidney cells

Elisabeth Holen, Hernandez Pedro W Araujo, Pål Asgeir Olsvik
2011

(Dietary) increase in ARA alters mineralization and PGE2 production

Miriam Furné Castillo, Hernandez Pedro W Araujo, Elisabeth Holen, Kvalheim Kvalheim, Kai Kristoffer Lie, Mari Moren
2000

Allergimedisin svekker ikke immunforsvaret

Elisabeth Holen, Thien van Do, Said Elsayed

Poster

2014

Bruk av LC-MS/MS for å karakterisere bioaktive peptider i Hpro®

Fiona Provan, Kjell Birger Øysæd, Marit Espe, Elisabeth Holen, Josef Daniel Rasinger, Eivind Larssen, Jørgen Seliussen
2008

A rapid method for the determination of prostaglandin E2 in cell cultures by liquid chromatography mass spectrometry

Hernandez Pedro W Araujo, Zhen-yu Du, Elisabeth Holen, Thu Thao Nguyen
2002

Dietary nucleotides and human immune cells

Elisabeth Holen, Oddvin Bjørge, Roland Jonsson

Rapporter og avhandlinger

2021

Trenger laks fettsyren EPA for optimal immunrespons? Rapport FHF prosjekt EPA-laks

Nina Sylvia Liland, Nini Sissener, Tone-Kari K Østbye, Tor Gjøen, Marta Bou, Elisabeth Holen, Bente Synnøve Ruyter, Aleksei Krasnov, Esmail Lutfi, Marit Espe, Ingunn Stubhaug, Grethe Rosenlund
2016

Effekter av endret fettsyresammensetning i fôr til laks relatert til fiskens helse, velferd og robusthet (Fett for fiskehelse): Oppdatering 2016

Nini Sissener, Bente Elisabeth Torstensen, Bente Ruyter, Tone-Kari K Østbye, Rune Waagbø, Sven Martin Jørgensen, Bjarne Hatlen, Nina Sylvia Liland, Elisabeth Ytteborg, Øystein Sæle, Ida Rud, Sofie C. Remø, Turid Mørkøre, Jens-Erik Dessen, Kaja Skjerven, Trine Ytrestøyl, Elisabeth Holen, Gerd Marit Berge, Anne-Catrin Adam
2007

Screening for stress inducible selected heat shock gene and protein expression in embryonic stem cells isolated from cod (Gadus morhua) blastulas

Endre Steigum, Elisabeth Holen
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