Hopp til hovedteksten
Elin Sørhus_1

Elin Sørhus

Phone: 952 40 889
Visiting address: Nordnesgaten 50, 5005 Bergen

Works with

Lately I have continued the work from my PhD, and am studying the effect of oil pollution on the early life stages of both haddock and cod. The aim is to further investigate the underlying mechansims resulting in the observed abnormalities in these species. In addition I work in a project studying the maturation of salmon. Here I spesifically focus on vgll3, a gene involved in the maturation age of salmon.

 

Curriculum Vitae

Education:

2013-2015. University of Oslo (UiO), PhD molecular biology and ecotoxicology.

In my PhD project: "Crude oil and its high toxic effect on early life stages of Atlantic haddock - to the heart of the problem", I have worked with embryo and larvae of Atlantic haddock. During my PhD period I have been involved in exposure of embryos and larvae, performed sampling, microscopy, photography and analysis of phenotypic data, analyzed full transcriptome data from several treatments and development stages and performed quantified and localized appearance of specific genes by Real Time RT PCR and in situ hybridization, respectively. I have also been locating and annotating several genes in the newly sequenced haddock genome.

Main findings in the study was the unexpected sensitivity of the haddock embryo to dispersed crude oil due to accumulation of oil droplets to the sticky haddock eggshell resulting in enhanced and prolonged exposure. In the study I also describe four distinct oil induced phenotypes, cardiac and craniofacial abnormalities and defects in ionregulation and fluid balance and cholesterol synthesis and homeostasis. The initiating event resulting in these phenotypes were identified by a chemical-genetic approach, where gene expression changes were linked to the oil induced phenotypes. E.g. the data suggest that disruption of calcium cycling in cardiac and craniofacial muscle cells is interacting with control of gene expression and thus, results in the abnormal development of the heart and craniofacial structures. In addition, I describe normal development of early stages of Atlantic haddock (8 stages from 2 days post fertilisation to 7 days post hatching), linking gene expression to appearance of visible elements or cellular processes in model organisms.

2002-2005. University of Bergen (UiB), Master in Molecular biology.

In the master thesis: ”Studies on minispectrins: Expression in E. coli, purification and in vitro interaction studies”, I studied a miniversion of spectrin, also called minispectrin. I made a modified version of the protein, without a specific N-terminal tail. This was done to study the structural and functional qualities of the two versions of the minispectrin. Cloning, transformation of E.coli cells, expression of protein in E.coli, protein purification, analysis of macromolecular interaction by using BiacoreX and ultracentrifuge action pull down assay was methods used during the master study.

1999- 2002. Bergen University College (HiB). Bioengineer education

A bioengineer analyse human sample specimens (etc. blood), and diagnosis and treatment are dependent on the correctness of the analysis. Therefore,the main emphasis of the bioengineer education is quality assurance, and thus, the bioengineer education provide training that emphasises high accuarcy and high quality in any laboratory task

Work experience:

2016- Today. Senior engineer at the Institute of Marine Research (IMR)

2013- 2015. CEES-UiO/VISTA/IMR. PhD student

2005-2012. Senior engineer at the Institute of Marine Research (IMR)

2005. Dep. engineer at the Hightechnology center in Bergen

1999-2004. Sommerjob as bioengineer/bioingenieer assistent

At IMR I have worked in two research groups, Reproduction and growth and Health research group. Working for Repr. and growth I have detected the amount of gene expression by various hormones and receptors involved in the sex differentiation and maturation in salmon and cod. When working for the Health group, the work has involved studies around the NODA infection in cod and halibut, and studies on the characterization of the immune system in halibut. After returning from my three year leave (PhD period: Jan. 2013- Dec. 2015), I have continued my work on the effects of oil pollution on the early life stages of haddock and cod.

Methods used in my work at IMR have been RNA isolation, analysis of RNA sequencing data, genome analysis including identification of genes, phylogenetic analysis and syntheny analysis, protein and RNA isolation systems, cDNA synthesis followed by PCR or real time PCR, sequencing, western blotting, immune histochemistry, in situ hybridization, microscopy and some microarray. I have also been involved in sampling and dissection of fish

At the Hightechnology center in Bergen I continued my studies on minispectrin.

 

Publikasjoner:

Sørhus E, Incardona JP, Karlsen Ø, Linbo T, Sørensen L, Nordtug T, van der Meeren T, Thorbjørnsen M, Jentoft S, Edvardsen RB, Meier S (2016b) Effects of crude oil on haddock reveal roles for intracellular calcium cycling in craniofacial and cardiac development. Scientific Reports 6.

Sørhus E, Incardona JP, Furmanek T, Jentoft S, Meier S, Edvardsen RB (2016a) Developmental transcriptomics in Atlantic haddock: Illuminating pattern formation and organogenesis in non-model vertebrates. Developmental Biology 411:301-313.

Sørhus E, Edvardsen RB, Karlsen O, Nordtug T, van der Meeren T, Thorsen A, Harman C, Jentoft S, Meier S (2015) Unexpected interaction with dispersed crude oil droplets drives severe toxicity in atlantic haddock embryos. PLoS One 10:e0124376.

Haugen T, Almeida FFL, Andersson E, Bogerd J, Male R, Skaar KS, Schulz RW, Sorhus E, Wijgerde T, Taranger GL (2012) Sex differentiation in Atlantic cod (Gadus morhua L.): morphological and gene expression studies. Reproductive Biology and Endocrinology 10.

Patel S, Sorhus E, Fiksdal IU, Espedal PG, Bergh O, Rodseth OM, Morton HC, Nerland AH (2009) Ontogeny of lymphoid organs and development of IgM-bearing cells in Atlantic halibut (Hippoglossus hippoglossus L.). Fish & Shellfish Immunology 26:385-395.