I am currently working as a postdoc in the NRC project SALMOSTERILE. The project aims to target germ cell survival factors to produce sterile Atlantic salmon for aquaculture; this could solve challenges like precocious puberty and genetic introgression from farmed escapees to wild populations.
Keywords: Atlantic salmon, functional studies, microinjection, gene knockout, CRISPR/Cas9, RNA sequencing, transcriptomics, in situ hybridization, qPCR.
2009-2013:Doctor of Philosophy (PhD), University of Bergen. Title of PhD thesis: "Transcriptome dynamics in Atlantic cod (Gadus morhua L.) during oogenesis and early embryonic development and in embryos from cortisol treated females".
2007-2009: Master degree in Aquaculture, Univerity of Bergen. Title of master thesis: ”Quantification and localization of StAR in Atlantic cod ovaries”.
2003-2006: Bachelor degree in general biology, University of Bergen.
2013-2016: Postdoc, Institute of Marine Research, Bergen, Norway.
2013: Engineer, Institute of Marine Research, Bergen, Norway.
2009-2013: PhD student, Institute of Marine Research, Bergen Norway.
2009: Research technician, Institute of Marine Research, Research station Matre, Norway.
2008-2009: Technician, Norsk Kveite AS, Askøy, Norway.
2006-2007: Reserach technician, Institute of Marine Research, Bergen, Norway.
Wargelius A, Leininger S, Skaftnesmo KO, Kleppe L, Andersson E, Taranger GL, Schulz RW, Edvardsen RB (2016). Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon. Scientific Reports 6:21284.
Wargelius A, Furmanek T, Montfort J, Le Cam A, Kleppe L, Juanchich A, Edvardsen RB, Taranger GL, Bobe J (2015). A comparison between egg transcriptomes of cod and salmon reveals species-specific traits en eggs for each species. Molecular Reproduction and Development 82(5):397-404.
Kleppe L, Wargelius A, Johnsen H, Andersson E, Edvardsen RB (2015). Gonad specific genes in Atlantic salmon (Salmo salar L.): characterization of tdrd7-2, dazl-2, piwil1 and tdrd1 genes. Gene 560(2):217-25.
Edvardsen RB, Leininger S, Kleppe L, Skaftnesmo KO, Wargelius A (2014). Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation. PLoS One 9(9):e108622.
Kleppe L, Edvardsen RB, Furmanek T, Andersson E, Taranger GL, Wargelius A (2014). Global transcriptome analysis identifies regulated transcripts during oogenesis and early embryogenesis in Atlantic cod. Molecular Reproduction and Development 81(7):619-35.
Kleppe L, Karlsen Ø, Edvardsen RB, Norberg B, Andersson E, Taranger GL, Wargelius A (2013). Cortisol treatment of prespawning female cod affects cytogenesis related factors in eggs and embryos. General and Comparative Endocrinology 189:84-95.
Kleppe L, Edvardsen RB, Kuhl H, Malde K, Furmanek T, Drivenes Ø, Reinhardt R, Taranger GL, Wargelius A (2012). Maternal 3'UTRs: from egg to onset of zygotic transcription in Atlantic cod. BMC Genomics 13:443.