Elisabeth Holen

Forsker
Telefon: 95241633
Avdeling: Behov og velferd

Publikasjoner

Faglige foredrag

2016

In Vitro Fisk: Nye 3D modeller til ernæring og toksikologisk forskning.

Liv Ingeborg Rosvoll Søfteland, Elisabeth Holen, Marit Espe, Pål Asgeir Olsvik
2014

Kan arginintilsetning påvirke forbrenning av visceralt fett gjennom økt polyaminomsetning i laks?

Synne Marte Andersen, Elisabeth Holen, Ivar Rønnestad, Jan Erik Zerrahn, Richard Taylor, Anders Aksnes, Marit Espe
2013

Do diets with 1-5% EPA and DHA induce inflammation in Atlantic salmon?

Nina Sylvia Liland, Elisabeth Holen, Bente Elisabeth Torstensen, Nini Sissener, Grethe Rosenlund
2000

Allergimedisin svekker ikke immunforsvaret

Elisabeth Holen, Thien van Do, Said Elsayed

Vitenskapelige artikler (NVI)

2017

Choline supplementation increased total body lipid gain, while surplus methionine improved growth and amino acid retention in adult Atlantic salmon (Salmo salar)

Marit Espe, Synne Marte Andersen, Eva Veiseth-Kent, Ivar Rønnestad, Elisabeth Holen, Jens-Erik Zerrahn, Anders Aksnes
Aquaculture Nutrition 23 p. 1086-1094
2017

Phytosterols are not involved in the development of fatty liver in plant oil fed Atlantic salmon (Salmo salar) at high or low water temperature

Nini Sissener, Nina Sylvia Liland, Elisabeth Holen, Ingunn Stubhaug, Bente Elisabeth Torstensen, Grethe Rosenlund
Aquaculture 480 p. 123-134
2016

Choline supplementation to low methionine diets increase phospholipids in Atlantic salmon, while taurine supplementation had no effects on phospholipid status, but improved taurine status

Marit Espe, Jens-Erik Zerrahn, Elisabeth Holen, Ivar Rønnestad, Eva Veiseth-Kent, Anders Aksnes
Aquaculture Nutrition 22 p. 776-785
2016

β-naphthoflavone interferes with cyp1c1, cox2 and IL-8 gene transcription and leukotriene B4 secretion in Atlantic cod (Gadus morhua) head kidney cells during inflammation

Elisabeth Holen, Pål Asgeir Olsvik
Fish and Shellfish Immunology 54 p. 128-134
2016

Hydrolyzed fish proteins modulates both inflammatory and antioxidant gene expression as well as protein expression in a co culture model of liver and head kidney cells isolated from Atlantic salmon (Salmo salar)

Elisabeth Holen, Juyun He, Pedro Araujo, Jørgen Seliussen, Marit Espe
Fish and Shellfish Immunology 54 p. 22-29
2015

Adult Atlantic salmon (Salmo salar L.) adapts to long-term surplus dietary arginine supplementation

Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe
Aquaculture Nutrition 21 p. 355-363
2015

Combining eicosapentaenoic acid, decosahexaenoic acid and arachidonic acid, using a fully crossed design, affect gene expression and eicosanoid secretion in salmon head kidney cells invitro

Elisabeth Holen, Juyun He, Marit Espe, Liqiou Chen, Pedro Araujo
Fish and Shellfish Immunology 45 p. 695-703
2015

Hydrolyzed fish proteins reduced activation of caspase-3 in H 2 O 2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar)

Marit Espe, Elisabeth Holen, Juyun He, Fiona Provan, Liqiao Chen, Kjell Birger Øysæd, Jørgen Seliussen
SpringerPlus 4
2014

The impact of exogenous ω-6 and ω-3 polyunsaturated fatty acids on the induced production of pro- and anti-inflammatory prostaglandins and leukotrienes in Atlantic salmon head kidney cells using a full factorial design and LC-MS/MS

Pedro Araujo, Eva Lucena, Yang Yang, Bupesh Ceemala, Zebasil Tassew Mengesha, Elisabeth Holen
Journal of chromatography. B 964 p. 164-171
2014

A co culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling

Elisabeth Holen, Marit Espe, Synne Marte Andersen, Richard Taylor, Anders Aksnes, Zebasil Tassew Mengesha, Pedro Araujo
Fish and Shellfish Immunology 37 p. 286-298
2014

Arginine supplementation and exposure time affects polyamine and glucose metabolism in primary liver cells isolated from Atlantic salmon

Synne Marte Andersen, Richard Taylor, Elisabeth Holen, Anders Aksnes, Marit Espe
Amino Acids 46 p. 1225-1233
2014

Aryl hydrocarbon receptor protein and Cyp1A1 gene induction by LPS and phenanthrene in Atlantic cod (Gadus morhua) head kidney cells

Elisabeth Holen, Pål Asgeir Olsvik
Fish and Shellfish Immunology 40 p. 384-391
2014

Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon

Marit Espe, Synne Marte Andersen, Elisabeth Holen, Ivar Rønnestad, Eva Veiseth-Kent, Jens-Erik Zerrahn, Anders Aksnes
British Journal of Nutrition 112 p. 1274-1285
2013

Cytokine gene expression and prostaglandin production in head kidney leukocytes isolated from Atlantic cod (Gadus morhua) added different levels of arachidonic acid and eicosapentaenoic acid

Miriam Furné Castillo, Elisabeth Holen, Pedro Araujo, Kai Kristoffer Lie, Mari Moren
Fish and Shellfish Immunology 34 p. 770-777
2013

Taurine attenuates apoptosis in primary liver cells isolated from Atlantic salmon (Salmo salar)

Marit Espe, Elisabeth Holen
British Journal of Nutrition 110 p. 20-28
2013

Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar)

Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe
British Journal of Nutrition 110 p. 1968-1977
2012

Low impact of exposure to environmentally relevant doses of Ra-226 in Atlantic cod (Gadus morhua) embryonic cells

Pål Asgeir Olsvik, Marc Berntssen, Ketil Hylland, Dag Ø. Eriksen, Elisabeth Holen
Journal of Environmental Radioactivity 109 p. 84-93
2012

Pathogen recognition and mechanisms in Atlantic cod (Gadus morhua) head kidney cells Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes

Elisabeth Holen, Kai Kristoffer Lie, Hernandez Pedro W. Araujo, Pål Asgeir Olsvik
Fish and Shellfish Immunology 33 p. 267-276
2012

Application of Doehlert uniform shell designs for selecting optimal amounts of internal standards in the analysis of prostaglandins and leukotrienes by liquid chromatography-tandem mass spectrometry

Hernandez Pedro W. Araujo, Steve Janagap, Elisabeth Holen
Journal of Chromatography A 1260 p. 102-110
2011

Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon (Salmo salar) fed soy bean oil or fish oil based diets

Elisabeth Holen, Synnøve Winterthun, ZHEN-YU DU, Anne Vatland Krøvel
Fish and Shellfish Immunology 30 p. 397-405
2011

Ontogenetic expression of maternal and zygotic genes in Atlantic cod embryos under ambient and thermally stressed conditions

Kaja Helvik Skjærven, Pål Asgeir Olsvik, Roderick Nigel Finn, Elisabeth Holen, Kristin Hamre
Comparative Biochemistry and Physiology A 159 p. 196-205
2011

Development of a co-culture model for in vitro toxicological studies in Atlantic salmon

Anne Vatland Krøvel, Synnøve Winterthun, Elisabeth Holen, Pål Asgeir Olsvik
Toxicology in Vitro 25 p. 1143-1152
2011

Transcriptional evidence for low contribution of oil droplets to acute toxicity from dispersed oil in first feeding Atlantic cod (Gadus morhua) larvae

Pål Asgeir Olsvik, Bjørn Henrik Hansen, Trond Nordtug, Mari Moren, Elisabeth Holen, Kai Kristoffer Lie
154 p. 333-345
2010

Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2

Elisabeth Holen, Anette Kausland, Kaja Helvik Skjærven
36 p. 1029-1039
2010

Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua) - Effects of BNF, PCDD and Cd

Liv Ingeborg Rosvoll Søfteland, Elisabeth Holen, Pål Asgeir Olsvik
151 p. 401-411
2007

Pigmentation and eye-migration in Atlantic halibut (Hippoglossus hippoglossus L.) larvae: Relevance of knowledge on development of pigment cells and determination of body axes in vertebrates.

Kristin Hamre, Elisabeth Holen, Mari Moren
Aquaculture Nutrition 13 p. 65-80
2005

Dietary nucleotides and human immune cells. II. Modulation of PBMC growth and cytokine secretion

Elisabeth Holen, Oddvin Anton Bjørge, Roland Jonsson
Nutrition Research 21 p. 1003-1009
2004

Dietary nucleotides and intestinal cell lines: I. Modulation of growth

Elisabeth Holen, Roland Jonsson
Nutrition Research 24 p. 197-207
1999

Horfor øker hyppigheten av allergi i industrialiserte land mer enn i utviklingsland?

Elisabeth Holen, Said Elsayed
? 119 p. 3176-3177
1999

Evaluating B and T cell epitopes of ovomucoid: Intact modified molecules, CNBr fragments and synthetic peptides

Said Elsayed, Elisabeth Holen
Allergy. European Journal of Allergy and Clinical Immunology 54 p. 211
1999

Characterization of T cell response to a-lactalbumin in PMMC cultures from cow mild hypersensitivity adults

Said Elsayed, Elisabeth Holen
Allergy. European Journal of Allergy and Clinical Immunology 54 p. 422

Annet tidsskriftbidrag

2013

Enhanced polyamine turnover after arginine supplementation in an Atlantic salmon model-implications on growth and animal welfare

Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe
Amino Acids 45 p. 575-576
2013

Methionine limitation-a threat to health? Using Atlantic salmon as the model

Marit Espe, Synne Marte Andersen, Elisabeth Holen, Jens-Erik Zerrahn, Ivar Rønnestad, E. Veiseth, Anders Aksnes
Amino Acids 45 p. 591-591

Rapporter og avhandlinger

2016

Effekter av endret fettsyresammensetning i fôr til laks relatert til fiskens helse, velferd og robusthet (Fett for fiskehelse): Oppdatering 2016

Nini Sissener, Bente Elisabeth Torstensen, Bente Ruyter, Tone-Kari K Østbye, Rune Waagbø, Sven Martin Jørgensen, Bjarne Hatlen, Nina Sylvia Liland, Elisabeth Ytteborg, Øystein Sæle, Ida Rud, Sofie C. Remø, Turid Mørkøre, Jens-Erik Dessen, Kaja Skjerven, Trine Ytrestøyl, Elisabeth Holen, Gerd Marit Berge, Anne-Catrin Adam
2007

Screening for stress inducible selected heat shock gene and protein expression in embryonic stem cells isolated from cod (Gadus morhua) blastulas

Endre Steigum, Elisabeth Holen

Poster

2014

Bruk av LC-MS/MS for å karakterisere bioaktive peptider i Hpro®

Fiona Provan, Kjell Birger Øysæd, Marit Espe, Elisabeth Holen, Josef Daniel Rasinger, Eivind Larssen, Jørgen Seliussen
2002

Dietary nucleotides and human immune cells

Elisabeth Holen, Oddvin Bjørge, Roland Jonsson

Jeg er senior forsker i Akvakultur Programmet: Behov og Velferd og arbeider med fiskevelferd, utvikler cellemodeller for å studere ernærings effekter på fiskehelse/velferd, alternative forkilder-, miljøgifter – fiskehelse, samt interaksjoner. Særlig fokus på  inflammasjon; mekanismer og signalveier, gener og protein markører.



Utvalgte publikasjoner
Prosjekter
Vitenskaplige publikasjoner


Utvalgte publikasjoner:

Elisabeth Holen, Marit Espe , Synne M. Andersen,  Richard Taylor, Zebasil Tassew Mengesha, Pedro Araujo. A coculture approach show that polyamine turnover is affected during inflammation in salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling.  Fish and Shellfish Immunology, 2014, 37, 286-298.

Pedro Araujo, Eva Lucena, Yang Yang, Bupesh Ceemala, Zebasil Mengesha, Elisabeth Holen. The impact of exogenous omega-6 and omega 3-polyunsaturated fatty acids on the induced production of pro- and anti-inflammatory prostaglandins and leukotrienes in Atlantic salmon head kidney cells using a full factorial design and LC-MS/MS. Chromatography B, 2014, http://dx.doi.org/10.1016/j.chromb.2014.01.018 .

E. Holen, KK Lie, P. Araujo, PA Olsvik. Pathogen recognition and mechanisms in Atlantic cod ( Gadus morhua) head kidney cells. Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes. Fish and Shellfish Immunology, doi:10.1016/j.fsi.2012.05.013.

Miriam Furne a,b , Elisabeth Holen a , Pedro Araujo a , Kai Kristoffer Lie a , Mari Moren a * Cytokine gene expression and prostaglandin production in head kidney leukocytes isolated from Atlantic cod ( Gadus morhua ) added different levels of arachidonic acid and eicosapentaenoic acid. Fish and Shellfish Immunology.  DOI: 10.1016/j.fsi.2012.11.044

E. Holen, S. Winterthun, Z.Y. Du, A. Krøvel. Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon ( Salmo salar ) fed soy bean oil or fish oil based diets. Fish and Shellfish Immunology. 30, 397-405, 2011.

E. Holen, A. Kausland,  K. Skjærven.  Embryonic stem cells isolated from Atlantic cod ( Gadus morhua ) and the developmental expression of a stage specific transcription factor ac-Pou2. Fish Physiol Biochem 2010, DOI 10.1007/s10695-010-9381-z

Olsvik PA & Holen E. Characterization of an Atlantic cod ( Gadus morhua ) embryonic stem cell cDNA library. BMC Research Notes 2:74, 2009

E. Holen, K, Hamre. Towards obtaining long term embryonic stem cell like cultures from a marine flatfish, Scopthalmus maximus. Fish Physiol Biochem 2004;  29:245-252.

  • 2015


    Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe

    Adult Atlantic salmon (Salmo salar L.) adapts to long-term surplus dietary arginine supplementation

    Aquaculture Nutrition; Volum 21.(3) S. 355-363
    • År: 2015

    Elisabeth Holen, Juyun He, Marit Espe, Liqiou Chen, Pedro Araujo

    Combining eicosapentaenoic acid, decosahexaenoic acid and arachidonic acid, using a fully crossed design, affect gene expression and eicosanoid secretion in salmon head kidney cells invitro

    Fish and Shellfish Immunology; Volum 45.(2) S. 695-703
    • År: 2015

    Marit Espe, Elisabeth Holen, J He, Fiona Provan, L. Chen, Kjell Birger Øysæd, Jørgen Seliussen

    Hydrolyzed fish proteins reduced activation of caspase-3 in H 2 O 2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar)

    SpringerPlus; Volum 4.(658)
    • År: 2015
  • 2014


    Marit Espe, Synne Marte Andersen, Elisabeth Holen, Ivar Rønnestad, Eva Veiseth-Kent, Jens-Erik Zerrahn, Anders Aksnes

    Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon

    British Journal of Nutrition; Volum 112. S. 1274-1285
    • År: 2014

    Pedro Araujo, Eva Lucena, Yang Yang, Bupesh Ceemala, Zebasil Tassew Mengesha, Elisabeth Holen

    The impact of exogenous ω-6 and ω-3 polyunsaturated fatty acids on the induced production of pro- and anti-inflammatory prostaglandins and leukotrienes in Atlantic salmon head kidney cells using a full factorial design and LC-MS/MS

    Journal of chromatography. B; Volum 964. S. 164-171
    • År: 2014

    Elisabeth Holen, Marit Espe, Synne Marte Andersen, Richard Taylor, Anders Aksnes, Zebasil Tassew Mengesha, Pedro Araujo

    A co culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling

    Fish and Shellfish Immunology; Volum 37.(2) S. 286-298
    • År: 2014

    Synne Marte Andersen, Richard Taylor, Elisabeth Holen, Anders Aksnes, Marit Espe

    Arginine supplementation and exposure time affects polyamine and glucose metabolism in primary liver cells isolated from Atlantic salmon

    Amino Acids; Volum 46.(5) S. 1225-1233
    • År: 2014

    Elisabeth Holen, Pål Asgeir Olsvik

    Aryl hydrocarbon receptor protein and Cyp1A1 gene induction by LPS and phenanthrene in Atlantic cod (Gadus morhua) head kidney cells

    Fish and Shellfish Immunology; Volum 40.(2) S. 384-391
    • År: 2014
  • 2013


    Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe

    Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar)

    British Journal of Nutrition; Volum 110.(11) S. 1968-1977
    • År: 2013

    Miriam Furné Castillo, Elisabeth Holen, Pedro Araujo, Kai Kristoffer Lie, Mari Moren

    Cytokine gene expression and prostaglandin production in head kidney leukocytes isolated from Atlantic cod (Gadus morhua) added different levels of arachidonic acid and eicosapentaenoic acid

    Fish and Shellfish Immunology; Volum 34.(3) S. 770-777
    • År: 2013

    Marit Espe, Elisabeth Holen

    Taurine attenuates apoptosis in primary liver cells isolated from Atlantic salmon (Salmo salar)

    British Journal of Nutrition; Volum 110.(1) S. 20-28
    • År: 2013
  • 2012


    Pål Asgeir Olsvik, Marc Berntssen, Ketil Hylland, Dag Ø. Eriksen, Elisabeth Holen

    Low impact of exposure to environmentally relevant doses of Ra-226 in Atlantic cod (Gadus morhua) embryonic cells

    Journal of Environmental Radioactivity; Volum 109. S. 84-93
    • År: 2012

    Elisabeth Holen, Kai Kristoffer Lie, Hernandez Pedro W. Araujo, Pål Asgeir Olsvik

    Pathogen recognition and mechanisms in Atlantic cod (Gadus morhua) head kidney cells Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes

    Fish and Shellfish Immunology; Volum 33.(2) S. 267-276
    • År: 2012

    Hernandez Pedro W. Araujo, Steve Janagap, Elisabeth Holen

    Application of Doehlert uniform shell designs for selecting optimal amounts of internal standards in the analysis of prostaglandins and leukotrienes by liquid chromatography-tandem mass spectrometry

    Journal of Chromatography A; Volum 1260. S. 102-110
    • År: 2012
  • 2011


    Elisabeth Holen, Synnøve Winterthun, ZHEN-YU DU, Anne Vatland Krøvel

    Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon (Salmo salar) fed soy bean oil or fish oil based diets

    Fish and Shellfish Immunology; Volum 30.(1) S. 397-405
    • År: 2011

    Kaja Helvik Skjærven, Pål Asgeir Olsvik, Roderick Nigel Finn, Elisabeth Holen, Kristin Hamre

    Ontogenetic expression of maternal and zygotic genes in Atlantic cod embryos under ambient and thermally stressed conditions

    Comparative Biochemistry and Physiology A; Volum 159.(2) S. 196-205
    • År: 2011

    Anne Vatland Krøvel, Synnøve Winterthun, Elisabeth Holen, Pål Asgeir Olsvik

    Development of a co-culture model for in vitro toxicological studies in Atlantic salmon

    Toxicology in Vitro; Volum 25.(5) S. 1143-1152
    • År: 2011

    Pål Asgeir Olsvik, Bjørn Henrik Hansen, Trond Nordtug, Mari Moren, Elisabeth Holen, Kai Kristoffer Lie

    Transcriptional evidence for low contribution of oil droplets to acute toxicity from dispersed oil in first feeding Atlantic cod (Gadus morhua) larvae

    Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology; Volum 154.(4) S. 333-345
    • År: 2011
  • 2010


    Elisabeth Holen, Anette Kausland, Kaja Helvik Skjærven

    Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2

    Fish Physiology & Biochemistry; Volum 36. S. 1029-1039
    • År: 2010

    Liv Ingeborg Rosvoll Søfteland, Elisabeth Holen, Pål Asgeir Olsvik

    Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua) - Effects of BNF, PCDD and Cd

    Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology; Volum 151.(4) S. 401-411
    • År: 2010
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