Elisabeth Holen

Seniorforsker

Telefon: +4795241633
Avdeling: Fiskeernæring,Behov og velferd

Jeg er senior forsker i Akvakultur Programmet: Behov og Velferd og arbeider med fiskevelferd, utvikler cellemodeller for å studere ernærings effekter på fiskehelse/velferd, alternative forkilder-, miljøgifter – fiskehelse, samt interaksjoner. Særlig fokus på  inflammasjon; mekanismer og signalveier, gener og protein markører.



Utvalgte publikasjoner
Prosjekter
Vitenskaplige publikasjoner


Utvalgte publikasjoner:

Elisabeth Holen, Marit Espe , Synne M. Andersen,  Richard Taylor, Zebasil Tassew Mengesha, Pedro Araujo. A coculture approach show that polyamine turnover is affected during inflammation in salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling.  Fish and Shellfish Immunology, 2014, 37, 286-298.

Pedro Araujo, Eva Lucena, Yang Yang, Bupesh Ceemala, Zebasil Mengesha, Elisabeth Holen. The impact of exogenous omega-6 and omega 3-polyunsaturated fatty acids on the induced production of pro- and anti-inflammatory prostaglandins and leukotrienes in Atlantic salmon head kidney cells using a full factorial design and LC-MS/MS. Chromatography B, 2014, http://dx.doi.org/10.1016/j.chromb.2014.01.018 .

E. Holen, KK Lie, P. Araujo, PA Olsvik. Pathogen recognition and mechanisms in Atlantic cod ( Gadus morhua) head kidney cells. Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes. Fish and Shellfish Immunology, doi:10.1016/j.fsi.2012.05.013.

Miriam Furne a,b , Elisabeth Holen a , Pedro Araujo a , Kai Kristoffer Lie a , Mari Moren a * Cytokine gene expression and prostaglandin production in head kidney leukocytes isolated from Atlantic cod ( Gadus morhua ) added different levels of arachidonic acid and eicosapentaenoic acid. Fish and Shellfish Immunology.  DOI: 10.1016/j.fsi.2012.11.044

E. Holen, S. Winterthun, Z.Y. Du, A. Krøvel. Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon ( Salmo salar ) fed soy bean oil or fish oil based diets. Fish and Shellfish Immunology. 30, 397-405, 2011.

E. Holen, A. Kausland,  K. Skjærven.  Embryonic stem cells isolated from Atlantic cod ( Gadus morhua ) and the developmental expression of a stage specific transcription factor ac-Pou2. Fish Physiol Biochem 2010, DOI 10.1007/s10695-010-9381-z

Olsvik PA & Holen E. Characterization of an Atlantic cod ( Gadus morhua ) embryonic stem cell cDNA library. BMC Research Notes 2:74, 2009

E. Holen, K, Hamre. Towards obtaining long term embryonic stem cell like cultures from a marine flatfish, Scopthalmus maximus. Fish Physiol Biochem 2004;  29:245-252.

  • 2015


    Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe

    Adult Atlantic salmon (Salmo salar L.) adapts to long-term surplus dietary arginine supplementation

    Aquaculture Nutrition; Volum 21.(3) S. 355-363
    • År: 2015

    Elisabeth Holen, Juyun He, Marit Espe, Liqiou Chen, Pedro Araujo

    Combining eicosapentaenoic acid, decosahexaenoic acid and arachidonic acid, using a fully crossed design, affect gene expression and eicosanoid secretion in salmon head kidney cells invitro

    Fish and Shellfish Immunology; Volum 45.(2) S. 695-703
    • År: 2015

    Marit Espe, Elisabeth Holen, J He, Fiona Provan, L. Chen, Kjell Birger Øysæd, Jørgen Seliussen

    Hydrolyzed fish proteins reduced activation of caspase-3 in H 2 O 2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar)

    SpringerPlus; Volum 4.(658)
    • År: 2015
  • 2014


    Marit Espe, Synne Marte Andersen, Elisabeth Holen, Ivar Rønnestad, Eva Veiseth-Kent, Jens-Erik Zerrahn, Anders Aksnes

    Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon

    British Journal of Nutrition; Volum 112. S. 1274-1285
    • År: 2014

    Pedro Araujo, Eva Lucena, Yang Yang, Bupesh Ceemala, Zebasil Tassew Mengesha, Elisabeth Holen

    The impact of exogenous ω-6 and ω-3 polyunsaturated fatty acids on the induced production of pro- and anti-inflammatory prostaglandins and leukotrienes in Atlantic salmon head kidney cells using a full factorial design and LC-MS/MS

    Journal of chromatography. B; Volum 964. S. 164-171
    • År: 2014

    Elisabeth Holen, Marit Espe, Synne Marte Andersen, Richard Taylor, Anders Aksnes, Zebasil Tassew Mengesha, Pedro Araujo

    A co culture approach show that polyamine turnover is affected during inflammation in Atlantic salmon immune and liver cells and that arginine and LPS exerts opposite effects on p38MAPK signaling

    Fish and Shellfish Immunology; Volum 37.(2) S. 286-298
    • År: 2014

    Synne Marte Andersen, Richard Taylor, Elisabeth Holen, Anders Aksnes, Marit Espe

    Arginine supplementation and exposure time affects polyamine and glucose metabolism in primary liver cells isolated from Atlantic salmon

    Amino Acids; Volum 46.(5) S. 1225-1233
    • År: 2014

    Elisabeth Holen, Pål Asgeir Olsvik

    Aryl hydrocarbon receptor protein and Cyp1A1 gene induction by LPS and phenanthrene in Atlantic cod (Gadus morhua) head kidney cells

    Fish and Shellfish Immunology; Volum 40.(2) S. 384-391
    • År: 2014
  • 2013


    Synne Marte Andersen, Elisabeth Holen, Anders Aksnes, Ivar Rønnestad, Jens-Erik Zerrahn, Marit Espe

    Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar)

    British Journal of Nutrition; Volum 110.(11) S. 1968-1977
    • År: 2013

    Miriam Furné Castillo, Elisabeth Holen, Pedro Araujo, Kai Kristoffer Lie, Mari Moren

    Cytokine gene expression and prostaglandin production in head kidney leukocytes isolated from Atlantic cod (Gadus morhua) added different levels of arachidonic acid and eicosapentaenoic acid

    Fish and Shellfish Immunology; Volum 34.(3) S. 770-777
    • År: 2013

    Marit Espe, Elisabeth Holen

    Taurine attenuates apoptosis in primary liver cells isolated from Atlantic salmon (Salmo salar)

    British Journal of Nutrition; Volum 110.(1) S. 20-28
    • År: 2013
  • 2012


    Pål Asgeir Olsvik, Marc Berntssen, Ketil Hylland, Dag Ø. Eriksen, Elisabeth Holen

    Low impact of exposure to environmentally relevant doses of Ra-226 in Atlantic cod (Gadus morhua) embryonic cells

    Journal of Environmental Radioactivity; Volum 109. S. 84-93
    • År: 2012

    Elisabeth Holen, Kai Kristoffer Lie, Hernandez Pedro W. Araujo, Pål Asgeir Olsvik

    Pathogen recognition and mechanisms in Atlantic cod (Gadus morhua) head kidney cells Bacteria (LPS) and virus (poly I:C) signals through different pathways and affect distinct genes

    Fish and Shellfish Immunology; Volum 33.(2) S. 267-276
    • År: 2012

    Hernandez Pedro W. Araujo, Steve Janagap, Elisabeth Holen

    Application of Doehlert uniform shell designs for selecting optimal amounts of internal standards in the analysis of prostaglandins and leukotrienes by liquid chromatography-tandem mass spectrometry

    Journal of Chromatography A; Volum 1260. S. 102-110
    • År: 2012
  • 2011


    Elisabeth Holen, Synnøve Winterthun, ZHEN-YU DU, Anne Vatland Krøvel

    Inhibition of p38 MAPK during cellular activation modulate gene expression of head kidney leukocytes isolated from Atlantic salmon (Salmo salar) fed soy bean oil or fish oil based diets

    Fish and Shellfish Immunology; Volum 30.(1) S. 397-405
    • År: 2011

    Kaja Helvik Skjærven, Pål Asgeir Olsvik, Roderick Nigel Finn, Elisabeth Holen, Kristin Hamre

    Ontogenetic expression of maternal and zygotic genes in Atlantic cod embryos under ambient and thermally stressed conditions

    Comparative Biochemistry and Physiology A; Volum 159.(2) S. 196-205
    • År: 2011

    Anne Vatland Krøvel, Synnøve Winterthun, Elisabeth Holen, Pål Asgeir Olsvik

    Development of a co-culture model for in vitro toxicological studies in Atlantic salmon

    Toxicology in Vitro; Volum 25.(5) S. 1143-1152
    • År: 2011

    Pål Asgeir Olsvik, Bjørn Henrik Hansen, Trond Nordtug, Mari Moren, Elisabeth Holen, Kai Kristoffer Lie

    Transcriptional evidence for low contribution of oil droplets to acute toxicity from dispersed oil in first feeding Atlantic cod (Gadus morhua) larvae

    Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology; Volum 154.(4) S. 333-345
    • År: 2011
  • 2010


    Elisabeth Holen, Anette Kausland, Kaja Helvik Skjærven

    Embryonic stem cells isolated from Atlantic cod (Gadus morhua) and the developmental expression of a stage-specific transcription factor ac-Pou2

    Fish Physiology & Biochemistry; Volum 36. S. 1029-1039
    • År: 2010

    Liv Ingeborg Rosvoll Søfteland, Elisabeth Holen, Pål Asgeir Olsvik

    Toxicological application of primary hepatocyte cell cultures of Atlantic cod (Gadus morhua) - Effects of BNF, PCDD and Cd

    Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology; Volum 151.(4) S. 401-411
    • År: 2010